s = '2022-09-30 16-01';
In the InputFormat option for the datetime function, 'hh' refers to "Hour, 12-hour clock notation using two digits" of the date and time string. 'HH' refers to "Hour, 24-hour clock notation using two digits". See the description of the Format property on the datetime documentation page for the table of components that you can use in the values for InputFormat and Format.
dt = datetime(s, 'InputFormat', 'yyyy-MM-dd HH-mm')
You may want to include hh and a in the Format if you want to see am/pm.
dt.Format = 'yyyy-MM-dd hh-mm a'
