PivLAB errors in calculation

Hello,
I am doing some analysis in PivLab version 2.56. Does anyone know why PivLab might be giving me very wrong results? Im running flow at approximatley 80 m/s and im expecting to see the u velocity component to be around that but when i run the PivLab analysis im getting values around -400 m/s. Its greatly exaggerated and in the wrong direction. I have used PivLab in the past with the same calibration and filters and have had no issues, im not sure what the problem is here. Does anyone have any ideas?

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Hi, please post your images data and your settings (including info on the calibration numbers) here or in the PIVlab forum, then I can check.

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