Thank you for the clarification. There may be too many plots for the data to look nice, but the way I would do what you have described is as follows:
[file,path] = uigetfile('*.*'); %select the file
cd(path) %change directories
fileData = readmatrix(file); %read the file
xdata = fileData(:,1); %value for data in the first column. I assumed there were 44 data points for X
hold on %hold onto the plot so it doesn't overwrite it
for col = 2:size(fileData,2) - 1 %loop through all columns of data minus the y average
ydata = fileData(:,col); %value for data in the looped column.
plot(xdata,ydata,'o') %plot the first and looped column
end
y_avg = fileData(:,end); %last column of data
plot(xdata,y_avg,'-') %plot the x vs. the average y data
hold off %turn the hold off
