What method can I use for background subtraction before performing analysis on my image?
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Hi, i am exploring matlab methods for personal growth and i want to determine how to:
 perform background substraction 
contrast correction, gaussian filtering and thresholding and binarization for my image.
I have attached a demo m file and image and i get this error:
Unrecognized function or variable 'lowThreshold'.
Error in testfiltering (line 53)
mask = grayImage > lowThreshold & grayImage < highThreshold; 
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  Image Analyst
      
      
 2023-1-15
        You need to assign that value.  See the interactive thresholding program in my File Exchange for how to do that.
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  Image Analyst
      
      
 2023-1-16
				I don't know how to answer this.  If you started with an image and a background image, you would certainly know if your code did a background subtraction.  If you don't have the original image and the background image, then where did you get the image that you have?
"how do I know if the thresholidng i see matches the thresholding i want?"  I don't know what this means.  It MUST match the threshold you want.  If it was not what you wanted, then why did you accept it?  Obviously you're only going to accept a threshold that you want.  You're not going to accept a threshold that you do not want, of course.  That said, it may be that there is no threshold value that will give you a perfect segmentation just by thresholding alone.
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