Hello
You can always overlay things by summing them, say your data is a 3D stack and each layer is one of your frames,
a = zeros(50,50,6);
a(1:10,1:10,1) = 1;
a(11:20,11:20,2) = 1.2;
a(31:40,1:10,3) = 0.8;
a(1:10,31:40,4) = 2;
a(41:50,1:10,5) = 3;
a(1:10,41:50,6) = 0.5;
% add a line to show where each frame is
a(1:3,:,:)=0.5;
a(:,1:3,:)=0.5;
montage(a)
Now, I can add all the frames and show together
imagesc(sum(a,3));
colormap gray