Writing Multichannel large tif image into pyramidal OME-tiff

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Aditya Misra 2023-4-23

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https://ww2.mathworks.cn/matlabcentral/answers/1951583-writing-multichannel-large-tif-image-into-pyramidal-ome-tiff

评论： Éric Martineau 2025-6-19

I initially had several channels of a fluorescent image (13 channels) that I then assembled into 1 multipage tiff using the bigtiff writer in MATLAB (since it is 17000 pixel x 34000 pixel x 13 channels). However, because I would like to open it in Qupath, I need a pyramidal version, which would be offered by an OME-TIFF format. Is there a way I can read in my multichannel tiff image and save a pyramidal version that can then be opened in Qupath? Or any other alternative?

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Éric Martineau 2025-6-19

Hi,

Did you find an answer in the end? I want to do something similar and I can't find a solution. I want to perform a z projection of large, multi-channel, multi-plane images and send them in a format I can open with quPath.

Thanks.

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