Writing Multichannel large tif image into pyramidal OME-tiff

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Aditya Misra
Aditya Misra 2023-4-23
回答： Priyank Pandey 2023-5-18
I initially had several channels of a fluorescent image (13 channels) that I then assembled into 1 multipage tiff using the bigtiff writer in MATLAB (since it is 17000 pixel x 34000 pixel x 13 channels). However, because I would like to open it in Qupath, I need a pyramidal version, which would be offered by an OME-TIFF format. Is there a way I can read in my multichannel tiff image and save a pyramidal version that can then be opened in Qupath? Or any other alternative?

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Priyank Pandey
Priyank Pandey 2023-5-18
Hi Aditya,
MATLAB does not have built-in support for creating pyramidal OME-TIFF files directly.
You can use additional tools, such as the Bio-Formats library, to achieve this. Refer the link below to know more about the toolbox:
Bioformats Image Toolbox - File Exchange - MATLAB Central (mathworks.com)
Regards
Priyank

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