how can i detect round objects and remove other objects in an image using matlab?

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IMAGES{i} = imread(sprintf(Fimage,i));
result = cell(1,N);
result{i} = rgb2gray(IMAGES{i});
BW = im2bw(result{i}, .4);
se = strel('disk',11);
erodedBW = imdilate(BW,se);
imagesc(erodedBW)
bw=bwareaopen(erodedBW,50000);
%imshow(bw)
%hold
[B,L] = bwboundaries(bw,'noholes');
for k = 1:length(B)
boundary = B{k};
xr(i,k)=round(mean((boundary(:,2))));
yr(i,k)=round(mean((boundary(:,1))));
imgindex(i)=i;
end
st=regionprops( ~bw,'area','centroid','PixelIdxList');

采纳的回答

Image Analyst
Image Analyst 2015-3-30
It seemed to work for me:
clc; % Clear the command window.
close all; % Close all figures (except those of imtool.)
clear; % Erase all existing variables. Or clearvars if you want.
workspace; % Make sure the workspace panel is showing.
format long g;
format compact;
fontSize = 20;
rgbImage = imread('005.png');
grayImage = rgb2gray(rgbImage);
subplot(2,2,1);
imshow(grayImage);
title('Original Image', 'FontSize', fontSize);
% Enlarge figure to full screen.
set(gcf, 'Units', 'Normalized', 'OuterPosition', [0 0 1 1]);
% Threshold the image.
binaryImage = im2bw(grayImage, .4);
% Display the image.
subplot(2,2,2);
imshow(binaryImage)
title('Initial Binary Image', 'FontSize', fontSize);
% Dilate the image to enlarge the small blobs.
se = strel('disk',11);
binaryImage = imdilate(binaryImage,se);
subplot(2,2,3);
imshow(binaryImage)
title('Dilated Image', 'FontSize', fontSize);
% Label the blobs.
labeledImage = bwlabel(binaryImage);
measurements = regionprops(labeledImage,'Area','Perimeter');
% Do size filtering and roundness filtering.
% Get areas and perimeters of all the regions into single arrays.
allAreas = [measurements.Area]
allPerimeters = [measurements.Perimeter]
% Compute circularities.
circularities = allPerimeters.^2 ./ (4*pi*allAreas)
% Find objects that have "round" values of circularities.
maxAllowableArea = 50000;
keeperBlobs = circularities < 3 & allAreas < maxAllowableArea; % Whatever values you want.
% Get actual index numbers instead of a logical vector
% so we can use ismember to extract those blob numbers.
roundObjects = find(keeperBlobs);
% Compute new binary image with only the small, round objects in it.
binaryImage = ismember(labeledImage, roundObjects) > 0;
subplot(2,2,4);
imshow(binaryImage);
title('Final Image', 'FontSize', fontSize);
% Remeasure with this new segmentation.
% Label the blobs.
labeledImage = bwlabel(binaryImage);
measurements = regionprops(labeledImage,'Area','Perimeter', 'Centroid');
% Get areas and perimeters of all the regions into single arrays.
allAreas = [measurements.Area]
allPerimeters = [measurements.Perimeter]
allCentroids = [measurements.Centroid]
centroidX = allCentroids(1:2:end);
centroidY = allCentroids(2:2:end);
% Plot circles around them
hold on;
for k = 1 : length(centroidX);
plot(centroidX(k), centroidY(k), ...
'ro', 'MarkerSize', 20, 'LineWidth', 2);
end
What does "not working well" mean to you? What exactly do you want as an output of this routine? The size, location, what?????
  14 个评论
Image Analyst
Image Analyst 2015-3-30
Why not check if the light is on by attaching an Arduino to the LED leads rather than by using image analysis?

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更多回答(2 个)

Image Analyst
Image Analyst 2015-3-30
Calculate the circularities and filter based on that.
labeledImage = bwlabel(~bw);
st=regionprops(labeledImage,'area','Perimeter');
% Get areas and perimeters of all the regions into single arrays.
allAreas = [st.Area];
allPerimeters = [st.Perimeter];
% Compute circularities.
circularities = allPerimeters.^2 ./ (4*pi*allAreas);
% Find objects that have "round" values of circularities.
roundObjects = find(circularities < 4); % Whatever value you want.
% Compute new binary image with only the round objects in it.
binaryImage = ismember(labeledImage, roundObjects) > 0;
imshow(binaryImage);

Alexandra Holland
Alexandra Holland 2022-2-11
I adapted the code above. May not be too elegant but it worked for me (I am looking at mouse cells).
circ_thresh = 1.15; %default circularity of 1.2 is OK, 1.05 is very stringent
%try to exclude weird shapes based on circularity
mask_conn= bwconncomp(mask_bs,8); % you need this as input for function 'regionprops'
mask_RP=regionprops(mask_conn,'area','Perimeter','PixelIdxList');
allAreas = [mask_RP.Area];
allPerimeters = [mask_RP.Perimeter];
circularities = allPerimeters.^2 ./ (4*pi*allAreas);
roundObjectsIndex = find(circularities < circ_thresh); % Whatever value you want.
mask_bsTEMP = false(size(DAPI_flat, 1), size(DAPI_flat, 2)); % initialize the mask as black
mask_RP_sort = mask_RP(roundObjectsIndex,:);
for k = 1:numel(mask_RP_sort)
idx = mask_RP_sort(k).PixelIdxList;
mask_bsTEMP(idx) = true;
end
mask_bs5=mask_bsTEMP; %test circularity threshold circ_thresh
imshow(mask_bs5);
  1 个评论
Image Analyst
Image Analyst 2022-2-12
There is now a 'Circularity' option to ask regionprops() for so you don't have to compute it yourself, though it's the inverse of what we computed. It's
(4*Area*pi)./(Perimeter^2)

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