how to get the folded image of a binary mask image...

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i wanted to invert a image along its majorAxis, and get the region difference in upper and lower half region... please do help me...

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Image Analyst
Image Analyst 2015-5-8
There's nothing like that in MATLAB. You'll have to write it from lower level functions. Here's how: Use regionprops() to find the orientation. Then use imrotate to rotate the image down. Then it's a simple matter of getting the top half line and XORing it with the bottom half line. I've attached some demos that might help you.
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Image Analyst
Image Analyst 2015-5-11
Supply the original image so I can run your code. Also, what do you want to do if the outlines overlap because one is not always larger than the other side?
Image Analyst
Image Analyst 2015-5-11
Why are the other two not correct? Why do you think the line does not go along the major axis? Keep in mind that the major axis does not necessarily lie along a line between the two farthest points. Just think if the blob was shaped like an L or an F - where do you think the major axis would lie? Not along the line between the two farthest points.

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