Is it possible to numerically label a segmented image?

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Diondra 2015-6-23

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https://ww2.mathworks.cn/matlabcentral/answers/224973-is-it-possible-to-numerically-label-a-segmented-image

编辑： Adam Danz 2019-12-13

SanchezLab_WatershedMethod_ImageSegmentation_Colony1.m

I've written some code to segment, count, and analyze the properties of bacterial colonies in a petri dish. Now, I would like to use principal component analysis to visualize the diversity of bacterial strains present in a given sample. My current method yields the PCA and plots 2 components against each other and even allows me to identify outlier points by number; however, I currently have no way of knowing which colony in my image actually corresponds to number "112" for example. Does anyone know of a way to overlay the colony numbers onto the original image so that I can have a reference point to make sense of the PCA data? I've attached my code if that's helpful.

Thank you in advance!

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Adam Danz 2015-6-23

编辑：Adam Danz 2019-12-13

Hello Katalin, if you have the x,y coordinates of the colonies, you can use labelpoints.m found in the file exchange. This function labels large groups of points like this:

labelpoints(x_vector, y_vector, labels)

and it has an optional outlier parameter that will only label the outliers. But this won't be helpful if you don't have vectors of X,Y values.

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Answer 1

Katalin 2015-6-23

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在 MATLAB Online 中打开

Hello! Try this:

M = im2uint8(label/NUM);
imshow(M)
s = regionprops(M, 'Centroid');
hold on
for k = 1:numel(s)
    c = s(k).Centroid;
    text(c(1), c(2), sprintf('%d', k), ...
        'HorizontalAlignment', 'center', ...
        'VerticalAlignment', 'middle', 'Color', 'red', 'fontsize',12);
end
hold off