Hello, I try to measure the particles size distributions of microscope pictures using image analysis. Works great when using Watershed algorithms and performing a binary particle analysis in the end. Now I want to fit the particle size distribution to a reference sample where I know the "real" distribution. Many different pieces of commercial software are able to perform such a calibration or fitting to a reference analysis so I think it is no magic. ;-) I thought about “calibration-factors” for each size class but first experiments in EXCEL give me really odd results when analyzing different samples distribution. The factors “warp” distributions which are not very close to the reference. I think I must calculate a “fitting” to the distribution and shift that fitting but I have no idea where to start. Can someone give me a tip or hint for the best way to perform that task? Best regards, Paul
