Setting scale (pixel to mm)

Zahid Manzar

2016 2 17

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8 次查看（30 天）

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So I have an image taken by a microscope (cel.png) it has a scale bar and the cell is gray etc. I need to find the area of the cell. My code is

clear;
clc;
prompt = 'Enter filename (include extension name): ';
str = input(prompt,'s');
A = imread(str);
image(A)
%Thresholding the image to gray-scale
level = graythresh(A);
BW = im2bw(A,level);
imshow(BW)
%Determine image dimensions
size = size(BW);                   
xpixel = size(2);                   %image dimesnions in the x axis
ypixel = size(1);                   %image dimesnion in the y axis
wpixel = length(BW(BW==1))
bpixel = length(BW(BW==0))

The threshold (refer to thshld.png) us working, its counting the white and black pixels. However, now I need to figure a way so the scale bar doesn't get counted and also I need to find out how to make matlab get pixel to mm ratio using the scale bar.

Any suggestions or do I need to use imageJ to get the pixel to mm ratio.