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how to find second and third order derivative of an image along z-axis on different scale?

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PriTi 2016-4-24

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https://ww2.mathworks.cn/matlabcentral/answers/280689-how-to-find-second-and-third-order-derivative-of-an-image-along-z-axis-on-different-scale

i am working on image segmentation . i need to find second and third order derivative of image on different scale(0.65, 1.1, 2.5).

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