The variable is properly created some lines above:
tmp = bwboundaries(handles.binaryImage_okoli);
shape.thorax = tmp{1};
But this code is not called, if 'okraje_modelu.mat' is existing. Then:
if 2==exist ('okraje_modelu.mat')
load ('okraje_modelu')
disp('Okraje modelù byly naèteny.')
Reading the code you cannot predict, what the load command injects in the workspace. Therefore it is much clearer and safer, to catch the output:
Data = load ('okraje_modelu');
shape = data.shape;
Then you will get a useful error message in case of a missing variable.
You can use the debugger to examine, what's going on. Set a breakpoint in the first line of the callback and step through the code line by line. Check in the Workspace Browser, of the wanted variable occurres.
