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how can i create trainning set for pca in matlab?

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Hi all
i have a database for 49 person , 4 images/person . i need to form training set for pca by representing each image by a column contains its pixels positon .by the way my image are b/w so how can i do this in matlab ?
is the way of representing images based on their pixels locations is preferable or not? is it the only way that i can use in forming trainning set or there is another way???
thanks to all

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