Standard Method for Detection of Focused Areas in Microscope Images?

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OkinawaDolphin 2017-4-18

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https://ww2.mathworks.cn/matlabcentral/answers/335955-standard-method-for-detection-of-focused-areas-in-microscope-images

评论： Image Analyst 2017-4-19

Is there a gold standard for finding focused areas in images acquired by optical microscopes? Is there a contrast measure frequently used?

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Image Analyst 2017-4-18

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If the "in focus" regions have higher texture then you can find those regions with a texture filter like stdfilt() or convolve with a Laplacian kernel and then threshold to find the "high texture" regions.

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OkinawaDolphin 2017-4-19

Photoshop will not help me much. I want to implement algorithms that detect focused areas on my own or to integrate implementations in the software I am working on.

Image Analyst 2017-4-19

It was just FYI - that there is some justification for the approach I suggested because other apps are doing similar things. So, try stdfilt() for starters.

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