Quantifying cells with different membrane stains

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Yonatan Degefu
Yonatan Degefu 2017-7-18
编辑： Mukul Rao 2017-7-21
I am trying to quantify cells that express membrane stains. So far I have used a K-means clustering approach but it has not worked well. The images I work with have blue stain for the nucleus and a red stain or a green stain surrounding the nucleus.I want to sort those blue cells that express either a red or a green stain and be able to quantify them. Image example attached below.
Any help is appreciated.

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回答（1 个）

Mukul Rao
Mukul Rao 2017-7-21
编辑：Mukul Rao 2017-7-21
Hi,
You can try the L*a*b color space technique, the example discussed is very similar to your use case.

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