Quantifying cells with different membrane stains
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I am trying to quantify cells that express membrane stains. So far I have used a K-means clustering approach but it has not worked well. The images I work with have blue stain for the nucleus and a red stain or a green stain surrounding the nucleus.I want to sort those blue cells that express either a red or a green stain and be able to quantify them. Image example attached below.
Any help is appreciated.
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