Calling imshow can delete the previous axes handle (which is your handles.axes1 and thus your hScatter too).
The following examples shows how imshow deletes handles.
a = axes;
b = imshow(ones(100), 'parent', a); %Show white image
imshow(zeros(100)); %Show black image, BUT deletes previous handles!
a =
handle to deleted Axes
b =
handle to deleted Image
To avoid deleting handles, directly change 'CData' of the image handle.
a = axes;
b = imshow(ones(100), 'parent', a); %Show white image
set(b, 'CData', zeros(100)); %Show black image, but no handles are deleted
So in your code, instead of this:
set(imshow(depth, [0 outOfRange]), 'CData', depth) %Which deletes hScatter when imshow is called.
try this (assuming the image handle is named ImHandle):
set(ImHandle, 'CData', depth) %Doesn't delete any handle
You can also use two separate handles on the GUI, like handles.axes1 and handles.axes2. Store the scatter on handles.axes1 ( using scatter(handles.axes1, ...) ), and image on handles.axes2 ( using imshow(...,'Parent', handles.axes2) ). If you want the image/scatter to be at the same place, use the handles.axesN.Visible = 'on' or 'off' to show or hide the the correct axes in the GUI.
