Thanks Guillaume !!!!!. I am able to create template of sign and symbol using the following code
%CREATE TEMPLATES
one=imread('1.png');
one=imresize(one,[42 24]);
two=imread('2.png');
two=imresize(two,[42 24]);
three=imread('3.png');
three=imresize(three,[42 24]);
four=imread('4.png');
four=imresize(four,[42 24]);
five=imread('5.png');
five=imresize(five,[42 24]);
zero=imread('0.png');
zero=imresize(zero,[42 24]);
sign=imread('sign.png');
sign=imresize(sign,[42 24]);
number=[one two three four five zero ];
character=[number sign];
NewTemplates1=mat2cell(character,42,[24 24 24 24 24 24 24], 3);
save ('NewTemplates1','NewTemplates1')
clear all