Is it possible to write a double data matrix into TIFF images ( Movie)?

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Jaladhar Mahato
Jaladhar Mahato 2018-1-13
评论： Walter Roberson 2021-11-20
I have a 100X100X19 matrix in the attached Aniso file. Now I want to convert those double data into images ( Movie). Is it at all possible?
FYI: I have used MATLAB "Tiff" function but it supports only the int16 data.
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Walter Roberson
Walter Roberson 2018-11-24
Jaladhar Mahato:
The imwrite2tif code that you attached as the solution is the code I linked to back on January 14 2018, the day after you posted your question.
So far I still find no evidence that any program supports movies stored in TIFF format. ImageJ supports reading TIFF into a "stack" and supports animating a stack, but if you wanted to save that as a video you would need animated GIF or AVI in ImageJ itself, with some other possibilities such as QuickTime being available through plugins -- but not one of the plugins I found supports movies stored in TIFF format.
Jaladhar Mahato
Jaladhar Mahato 2018-11-25
编辑：Jaladhar Mahato 2018-11-25
Dear Walter Roberson,
You are right that You have provided me the link of that code date back to January 14, 2018. Actually, I was seeking this answer a long time ago and found it, but was not able to use it. After communicating with You I have found the trick to use it and that is why I have recommended your answer. However, I don't think a new user will be able to use this function properly. It is simple but complicated.
FYI: The default file format in ImageJ is Tiff only. We almost daily used ImageJ to read, write and store in a tiff movie file. I can say that ImageJ is made for tiff. The good thing about ImageJ is that You can visualize a single precision 3D data matrix as an image, which Matlab can represent in a complicated way. This helps a broad range of layman (in terms of image analysis) working in different field of microscopy and astronomy (few). However, You can not do any operation on 64-bit images in ImageJ, but you can open in Fiji- an ImageJ based java platform.
Regards
Jaladhar

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采纳的回答

Walter Roberson
Walter Roberson 2018-1-14
It is possible to write ieee single (32 bit) to tiff.
It is not possible to write in half precision (16 bit floating point) in any released version of MATLAB; I would need to check to see if that is even supported by the TIFF 6.0 standard. I do not recall support for half precision anywhere in any MATLAB toolbox.
But these matters are not relevant because TIFF has no movie format. If you need to create movies then you need to use a different format.
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Jaladhar Mahato
Jaladhar Mahato 2018-1-14
Dear Walter Roberson,
We have a data acquisition software where we can collect movie into 16bit Tiff format. We can open those images in a software name as ImageJ (NIH) and can do many image processing operation. But yes, I agree with You, when we do any operation on floating point, we converted the image into a 32-bit image.
Here in MATLAB, although I can do a hell lot of stuff, but I can not save the final result in a double precision Tiff format.
Thanks and Regards
Jaladhar Mahato

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更多回答（2 个）

Jan
Jan 2018-1-13
This will work with imwrite.
Data = load('Aniso.mat');
A = Data.A;
imwrite(A(:, :, 1), 'test.tiff');
for k = 2:size(A, 3)
imwrite(A(:, :, k), 'test.tiff', 'WriteMode', 'append');
end
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Jaladhar Mahato
Jaladhar Mahato 2018-1-14
My question was converting a double data matrix into TIFF format, whereas "Tiff" only work with int16, int32 unit16, unit32.
"imwrite" and "Tiff", convert my data into integer whereas my data have decimal values. I want exact input values in the output images (Movie). Hope You got my point.
Jan
Jan 2018-1-14
You can convert the decimal values to uint16 by:
data = rand(640, 480);
dataU16 = im2uint16(data);
This can be written for uint32 also:
dataU32 = data * intmax('uint32');
Of course you loos some precision, but TIFF files are not designed to store double data. As you see in Walter's answer, at least single would work.

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Alexandra Holland
Alexandra Holland 2021-11-19
I hope this can help some of you. I am not sure if this is answering your question though, but you can save your double as a .mat file, then load it, then extract it from the resulting structure as follows:
cd(folder_name)
save('file_name.mat','var_name');
X = load('file_name.mat');
Since you end up with a structure, you need to extract the data as follows:
Y = X.var_name; % voila. You have your double back.
You can check you end up with the exact same with the command
Z=mean(Y-var_name,'all'); % and you can verify that Z=0.

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