Read about xlsread . With this you can read excel data into matlab.
files = dir('*.xls') ; % Excel file extension
N = length(files) ; % total files
iwant = cell(N,1) ;
for i = 1:N
[num,txt,raw] = xlsread(files(i).name) ;
iwant{i} = num ;
end
Now you have all the data of excel files in iwant. You can write that into a single file.
