How can I extract the inner cell body(s) from the cell capsule in images (grayscale) such as these ??

Question

Gaurav Kudva 2018-2-5

0
链接

此问题的直接链接

https://ww2.mathworks.cn/matlabcentral/answers/380881-how-can-i-extract-the-inner-cell-body-s-from-the-cell-capsule-in-images-grayscale-such-as-these

回答： Arun Mathamkode 2018-2-22

This is what I have written so far but I cannot seem to get the exact shape of the cell body in the mask

image_file = 'IMAGE_LOCATION';                                                                                  
image = imread(image_file);                                                                                       
figure                                                                                                                   
imshow(image);                                                                                                            
image = adapthisteq(image);                                                                               
figure                                                                                                               
imshow(image);
image_wth = image - imopen(image, strel('disk', 5));                                                                
figure
imshow(image_wth);
image_bw = imbinarize(image_wth);                                                                             
image_bw = bwareaopen(image_bw, 20);                                                                              
image_bw = imclose(image_bw, strel('disk', 5));                                                                  
image_bw = imfill(image_bw, 'holes');                                                                                 
B = bwboundaries(image_bw);                                                                                               
figure
imshow(image_bw);                                                                                                    
hold on    
visboundaries(B); 
figure          
imshowpair(image_wth, image_bw); 
key = waitforbuttonpress;  
close all

1 个评论
显示 -1更早的评论隐藏 -1更早的评论

Gaurav Kudva 2018-2-6

编辑：Gaurav Kudva 2018-2-6

This is what the result looks like for the first image using the code given above.

But a part of the first cell body is missing in the mask.

So I need help in extracting the exact shape of the cell body in the mask. How do I go about it ??

请先登录，再进行评论。

请先登录，再回答此问题。

Answer 1

Arun Mathamkode 2018-2-22

0
链接

此回答的直接链接

https://ww2.mathworks.cn/matlabcentral/answers/380881-how-can-i-extract-the-inner-cell-body-s-from-the-cell-capsule-in-images-grayscale-such-as-these#answer_306466

To get good results you have to finetune the parameters in the algorithm. For example, giving a lower threshold for imbinarize function (may be 0.1) will give you complete mask. Although I am not much clear about the algorithm you are using, I believe it is heavily depended on multiple parameters. Tuning these parameters for different images will be difficult.

If we can assume the cell body will be approximately circular, we can use imfindcircles to detect the circles in the image and use imfill to create the mask from the circle boundaries. I believe this approach will be more robust.

0 个评论
显示 -2更早的评论隐藏 -2更早的评论

请先登录，再进行评论。

How can I extract the inner cell body(s) from the cell capsule in images (grayscale) such as these ??

1 个评论
显示 -1更早的评论隐藏 -1更早的评论

回答（1 个）

0 个评论
显示 -2更早的评论隐藏 -2更早的评论

另请参阅

类别

标签

Community Treasure Hunt

How can I extract the inner cell body(s) from the cell capsule in images (grayscale) such as these ??

1 个评论 显示 -1更早的评论隐藏 -1更早的评论

回答（1 个）

0 个评论 显示 -2更早的评论隐藏 -2更早的评论

另请参阅

类别

标签

Community Treasure Hunt

1 个评论
显示 -1更早的评论隐藏 -1更早的评论

0 个评论
显示 -2更早的评论隐藏 -2更早的评论