Color deconvolution for stain separation in biological images

Question

Tim 2012-5-14

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https://ww2.mathworks.cn/matlabcentral/answers/38293-color-deconvolution-for-stain-separation-in-biological-images

评论： Walid 2013-10-10

Hello,

I am using the Matlab Image Processing Toolbox to detect cells in biological images.

I have dual-stained images and I would like to apply color deconvolution (as described here: http://www.dentistry.bham.ac.uk/landinig/software/cdeconv/cdeconv.html) to separate the stains. So my input is an RGB image and I would like to get 3 gray scale output images: 1 for each of my stains and 3 third one for everything else (noise). I would then use thresholding on the first two images to detect objects in them.

I have already determined color vectors that describe my 2 stains from sample image regions.

Can anyone give some example code or advice on which functions to use in Matlab to get this done?

Thanks in advance,

Tim

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Answer 1

Manikanta Prasanth 2013-10-10

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https://ww2.mathworks.cn/matlabcentral/answers/38293-color-deconvolution-for-stain-separation-in-biological-images#answer_99218

Hello Tim. If you are still in need of a code, follow this link. http://web.hku.hk/~ccsigma/color-deconv/color-deconv.html

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Walid 2013-10-10

Thanks. I would like to use the algorithm to extract Hematoxylin vs DAB (brown staining). I have separate images with only Hematoxylin or only DAB. How can I do it ?

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