Detecting shapes-lsm format?

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Sameer Dixit
Sameer Dixit 2012-5-24
I want to detect the number of blue coloured spots (nuclei to be precise) in an image of lsm format using matlab, however writing the code below is not detecting the shapes at all, instead as i do imshow a white page opens up.(Indicating that the entire picture has been converted into white) code: i=imread('01.lsm'); imshow(i) BW = im2bw(i, 0.9); BW = ~BW; imshow(BW)
Is it a problem with the code or with the format? Thanx in advance.

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回答（1 个）

Stephen
Stephen 2012-5-24
try looking at hist(i,100) and picking out a threshold manually. somewhere to the right of the big background spike on the left.
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Stephen
Stephen 2012-5-24
oh and imclearborder() bw=bwareaopen() and imfill(bw,'holes') could be pretty useful for you too

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