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Detecting nuclei from a microscopic image

2 次查看（过去 30 天）
Sam Lee
Sam Lee 2018-4-7
关闭： John D'Errico 2018-4-7
I have these microscopic images from which I need to detect the nuclei. I sharpened the image using "localcontrast()" and the nuclei look pretty decent. After, I tried reducing noise using medfilt3() but most of the nuclei disappeared. How can I solve this problem?
Here is one of the images after sharpening (need to keep only the green spots):

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