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Background Subtraction

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Jay
Jay 2012-6-11
Hello!
I have an image of some fluorescent samples http://imageshack.us/photo/my-images/440/washingexp.png and I need to get a "intensity" reading from them. All is good except for the background subtraction in the image. I want to sum up my samples only and not the background and I am having trouble doing this.
Does anyone have any tips on background subtracting? Every time I do it MatLab cuts out from my samples. I really want to single out my samples but not lose them! :)
Thank you!
I'm doing something like
I =imread('washingexp.tif');
for r=1:1024
for C=1:1360
B(r,C)=I(r,C)-88;
end
end
  2 个评论
Ryan
Ryan 2012-6-11
Do you have the image processing toolbox.
Walter Roberson
Walter Roberson 2012-6-12
It is not immediately clear which parts are background or not? In particular it is not clear if the bright dots are part of the background or the foreground

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回答（1 个）

Ryan
Ryan 2012-6-11
There are various methods to better segment your image and most are best dealt with on a case by case basis.
Check out Image Analyst's tutorials on the file exchange: http://www.mathworks.com/matlabcentral/fileexchange/authors/31862
In particular this should be of help: http://www.mathworks.com/matlabcentral/fileexchange/25157-image-segmentation-tutorial-blobsdemo
  2 个评论
Ryan
Ryan 2012-6-11
If you have the image processing toolbox:
I = imread('imagefile.png");
BW = im2bw(I,graythresh(I)); % "graythresh(I)" is Otsu's method for finding the optimal intensity level for performing an image threshold.
BW2 = imfill(BW,'holes');
Labels = bwlabel(BW2,8);
Properties = regionprops(Labels,'all');
help regionprops
Ryan
Ryan 2012-6-11
use bwareaopen(Image,'200 or some other "area" value) to eliminate the glares if you need to.

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