need to find difference of two cells from one column in Excel

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Hi, I have an excel where column one(Time) has some values and column 5 (Duration), in duration i need to find the difference of 2nd element - 1st element from column one, i dont want to hard code that on excel, i want write that piece of code in .m file, how do i do it?
[num2, text, alldata2]=xlsread('exp2.xlsx');
temptime=num2(:,1); % getting column one from excel
holder=length(temptime); % getting to know how many elements are there in column one
i=1;
for i = 1:holder
duration()=(temptime(i)-temptime(i-1)); %its failing here
end
%% Excel is attached to this question

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ahmed nebli
ahmed nebli 2018-8-22
i think it should be done this way:
for i = 2:holder % the counter must get from 2 because temptime(i-1) would be error duration()=(temptime(i)-temptime(i-1)); %its failing here end

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