Image thresholding using min and max pixel intensity values

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Hi,
I'm trying to create a mask based on a minimum and maximum intensity value. All the examples I find are basically thresholding the image from a fixed value (say, 176) to 255. What I need to do is to mask pixels between 165 and 166 to find ROIs on the image.
Thanks!

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Image Analyst
Image Analyst 2018-9-3
Try this:
mask = grayImage >= 165 & grayImage <= 166 % Mask of only pixels with GLs of 165 and 166
grayImage(~mask) = 0; % blacken outside the mask.

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