Diameter droplet on image low contrast

Question

falguifrog 2019-1-12

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https://ww2.mathworks.cn/matlabcentral/answers/439441-diameter-droplet-on-image-low-contrast

评论： Kul Pun 2020-10-8

Dear all,

I have a bunch of pictures like as I uploaded in my post. The image represents lipids droplets of an adipocyte. The images are acquired on a Raman spectrometer with transmitting white light. The numbers in the lipid droplets show where I did my analysis. I cannot re-acquire the image since the samples are gone. What I would like to do? I would like to calculate the diameter of droplets (which has a number inside).

My problem is: it is difficult to segment the shape of the droplet based only on colour of the droplet, because there is no clear contrast.

I’m a beginner in the image processing toolbox, but I’m familiar with Matlab. I made some search on internet, but there is so many functions with the image processing toolbox that I feel lost.

My two questions to the community:

Could I calculate the diameters with Matlab with this kind of image ?or the image is so “bad” that I can’t calculate the diameter?
Could you give me tracks to follow because I don’t know where to start?

My plan B is to segment manually the droplet with Image J. But I have approximately 6000 droplets. Doing this manually and one by one, I cannot imagine to do this.

Thanks for your help !

PS: From my research on internet, I was thinking about morphological function. Is it the good track?

3 个评论
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Image Analyst 2019-1-12

It might take less time to manually outline 6000 droplets than to develop an automatic algorithm. I have an example attached that used imfreehand().

Kul Pun 2020-10-8

hi, i am really struggling to find the droplet of water and oil can u guys suggest me the best way to calculate the oil and droplet which may be 500-1000 um. pl

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