circle cells which are not so circular

Question

Lea Corbova 2019-4-26

1
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https://ww2.mathworks.cn/matlabcentral/answers/458713-circle-cells-which-are-not-so-circular

Hi everyone!

I am actually working on my bachelor project. I can currently detect nuclei of leucocytes, but I would like to compare number of nuclei with number of cells. How can I detect the number of leukocytes?

I tried to find circles in images with function: various radii in grayscale image via Hough Transform and imfincircles but it did not help me, I could detect number of cells just on one image and on the others it was not accurate, even when I added automatic threshold as one of the parameters for detecting circles.

I attached sample of images which I am working with and result of finding nuclei with K-means function :

This last image is result of imfindcircles , but it was accurate filter just for this image, for others it detected one or more circles without detecting real cells.

I used:

vybrany= imread('C:\Users\Lea\Documents\Bakalarka\komprimovane\100\Screenshot_45.jpg');
imshow(vybrany), title('targetImage');
x=rgb2gray(vybrany)
imshow(x)
threshold =graythresh(x)
[centersBright,radiiBright,metricBright] = imfindcircles(x,[35 105], ...
    'ObjectPolarity','dark','Sensitivity',0.96,'EdgeThreshold',(threshold./1.9));
viscircles(centersBright, radiiBright,'Color','b');