Hi, your image is not uploaded correctly.
Your case can be solved by segmenting the image to get the object of interest localized, in your cases foci-formation on nucleus. Image properties are very important for segmentation and decides the segmentation approach. Then we can perfrom blob detection on the segmented image which will give you the size and intensity information. You may also have to do some sort of morphological operation to remove the insignificant blobs. The steps in sequence are given below.
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