Otsu Thresholding for multi pixel value

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Kingsley Choong
Kingsley Choong 2019-11-5
model1b.jpg
Let say i have a grayscale retinal image like this which has three different pixel value which is 100 (background),130(vessel) and 70(dots). Can i only segment the 70 pixel of the dot out from the image to become a binary image by otsu thresholding method in matlab? If can, can pls provide some guidline or code? thank you for the help.

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