help with overlapping spots

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Mary Kodogianni 2011-4-7

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https://ww2.mathworks.cn/matlabcentral/answers/4996-help-with-overlapping-spots

关闭： MATLAB Answer Bot 2021-8-20

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hello everybody, I'm trying to solve the problem of overlapping gaussian spots,but i have problems with it.My table with spots takes values between 0-1 .I am writing the code below.The problem is that i take the minimum values and they are at about 46656 and they are zero values most of them and they don't belong to the spots but to the background that doesn't have importance for my algorithm.

function [lol,count,x1,y1,x2,y2,overlap,Z,L,B1]=avoid_overlap_6_4(z)
overlap=0;

%[x1,y1]=find(Z1~=0); %Z(x1,y1)=Z1(x1,y1); z=round(z*100000)/100000;

idx1=find(z~=(0)); Z(idx1)=z(idx1); B2= imregionalmax(Z,8); [x2,y2]=find(B2==1); BW=im2bw(Z); [L,NUM] = BWLABEL(BW,8); count=0; lol=1; B2= imregionalmax(Z,8); [x2,y2]=find(B2==1); for i=1:size(x2)-1 overlap(i)=0; end B1= imregionalmin(Z,8);

m=1; [x1,y1]=find(B1==1);

k=1;

t=1; m=1; for i=1:size(x2)-1 % if m<=NUM [r,c]=find(L==i); temp=Z(r,c); if ismember(Z(x2(i),y2(i)),temp) & ismember(Z(x2(i+1),y2(i+1)),temp)

       for k=1:size(x1)-1
           if Z(x1(k),y1(k))<Z(x1(k+1),y1(k+1)) 
                lol=4000;
          %     if Z(x1(k),y1(k))<Z(x2(i+1),y2(i+1))
            %   if ismember(Z(x1(k),y1(k)),temp)
                count=count+1;
              Z(x1(k),y1(k))=0;
              %      k=k+1;
                          %Z(s1(max(thesi)).x0,s2(max(thesi)).y0)=0
            %   end
           end
       end
    end