How can I write a 4 dimensional Tiff from a 4D matrix?

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Marcus Leiwe 2020-1-17

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https://ww2.mathworks.cn/matlabcentral/answers/500688-how-can-i-write-a-4-dimensional-tiff-from-a-4d-matrix

编辑： Walter Roberson 2020-5-25

I'm trying to convert 4d matrix (XYCZ) into a Tiff that will have also have the required metadata information such as the voxel resolution. I've tried looking at functions such as bfsave, from the bio-formats group but the file size is 4 times bigger (as well as generating issues when trying to apply the original metadata - extracted from bfopen - to the 4d matrix). Is there a faster way?

Ultimately I'm trying to generate an image that can be read by standard microscopy software such as Neurolucida, and FIJI/ImageJ

Thanks in advance,

Marcus

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Marcus Leiwe 2020-1-17

Thanks Walter,

Using the bioformats io plugin you are able to open it as a hyperstack. But as you said it does rely on the metadata. However, I'm having trouble correctly applying the metadata from the original image onto the image I've transformed in MATLAB.