how load a Neural network file with a push button and used it in other Pushbutton callback function???

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here is my code;
Plz tell me whats wrong: I am new to GUI (programming); i want to load a "net0" named varialble and use it in other callback finction "Rcrd_and_Recog".
Note: i am not using GUIDE GUI; using programming GUI.
function ASR()
figure('Name','Automatic Isolated Speech Rcognition System',.......
'Menubar','none',........
'Color',[1 1 1]);
Rcrd_and_Recog =uicontrol('Style','pushbutton',....
'Units','normalized',....
'Position',[0.75 0.75 0.20 0.05],....
'String','START',....
'Callback',@Record_Recog);
LD_net = uicontrol('Style','pushbutton',....
'Units','normalized',....
'Position',[0.75 0.65 0.20 0.05],....
'String','LOAD THE NET',....
'Callback',@load_net);
function load_net(varargin)
[file path]=uigetfile('*.mat','Select the M-file');
if ~isequal(file, 0)
L=load(fullfile(path,file));
Net=fieldnames(L);
net=Net{1};
handles.net=net;
end
function Record_Recog(varargin)
fs=16000;
y=wavrecord(1*fs,fs,1,'double');
if length(y)<1157
result=sim(net0,cat(1,y,zeros(1157-length(y),1)))
else
result=sim(net0,y)
end
end

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