Your question isn't quite clear. If you want to get fluctuations of subsequent points in a time series as you described above, you can use diff function to do the first order difference, or simply using locs(2:end) - locs(1:end-1) (locs in the variable you defined in your program).
The thing that is confusing is that you are trying to get RRI within a 1-s window. That doesn't make a lot of sense as usually the heart beats about once, or a bit more than twice per second when people exercise. You are not able to get any fluctuations from a single heart beat.
