Particle Tracking

Question

Scott G 2011-4-19

1
链接

此问题的直接链接

https://ww2.mathworks.cn/matlabcentral/answers/5838-particle-tracking

评论： Arash Beyrami 2020-1-24

I am doing some research and need to track the displacement of nanoparticles in a video that I captured. I have started to write the code; however, I am stuck.

I need to find a way to label each particle and determine in the next frame where that particle moved to. I have calculated the centroid of each particle and written them to a table. I am not sure how to compare these centroid particles and track them across 50 frames. Particles tend to come and go as they go in and out of the focal plane.

Thanks.

Code:

clear all; 
mov = aviread('50Mol_Simul.avi'); %Convert video to images
for m =1:length(mov)
 [im,map] = frame2im(mov(m));
 imwrite(im, ['im' num2str(m) '.jpg'],'jpg');
end 
mov = mmreader('50Mol_Simul.avi');
implay('50Mol_Simul.avi');
nFrames = mov.NumberOfFrames
Width = mov.Width
Height = mov.Height
for k = 1 : nFrames
  singleFrame = read(mov, k);
  level = graythresh(singleFrame);
  bw = im2bw(singleFrame, level);         
%     figure;
%     imshow(bw);
  bw = bwareaopen(bw, 3);
  L = bwlabel (bw,8);
  stats = regionprops(bw,'Centroid');
  V = cat(1,stats.Centroid);
  temp.ctrd = V;
  temp.index = zeros(size(V,1),1);
  a(k) = temp;
end