how to detect some difficult circles and count them

Question

Lucas Moreira 2020-10-8

0
链接

此问题的直接链接

https://ww2.mathworks.cn/matlabcentral/answers/608706-how-to-detect-some-difficult-circles-and-count-them

评论： Lucas Moreira 2020-10-15

i'm trying to count some colonies of bacterias and this is the result i got till now, i'm having problem at detecting them properly, i tried imfindcircles, but the range i pass detect a lot of false positives, can someone help me?

the code is:

clear variables;
close all;
warning off;
img = imread('colonies21.jpg');
imgcinza = rgb2gray(img);
equalized = adapthisteq(imgcinza);
limiar = graythresh(equalized);
imbin = imbinarize(equalized,limiar);
filled = imfill(~imbin,'holes');
imgjunta = (double(img)./255) .* filled;
imgjuntabin = rgb2gray(imgjunta) > 0.4;
imshow(imgjuntabin);