Hi,
i have a tif image stack from a 3D microscop. the stack contain a large number of cells, and i need to isolate every cell and eventually create a sub-stack for every cell found. i need to find the 3D connected components in the stack.
first thing is to process the images, clean them and turn them into a black and white stack so the CC will work.
i tried lots of combinations of morphological operations, hole filling, in 3D, in 2D (for every image instead of working on the stack itself), using filters and so on.
i cant get good enough result for the CC to work good enough.
i managed to clear the noise that was caused by light in the microscop, but cant clear it good enough.
attaching some images from the stack for better understanding of the data.
each stack is 300-320 images
any idea or help will be great!
ps - i dont have any option to improve my raw data.
thanks!
