How to use Lucas Kanade Function with image instead of video

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Does anyone ever tried to use the Lucas Kanade function to track object (in my case road signs) in successive images instead of a video^. I got a algorithm that works ok right now, but it doesn't track objects through my list of image.
Can someone please help me!!!!
I cant put my code in the forum cause there's too much different function in it. I would like to know how do you construct and initialized the Lucas Kanade function.
Thank you

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