Hi Gideon,
What you need to do is read all tiff file in MATLAB and convert them into a big array containing all the images. Check out read or imread function to read the tiff images. This should help you. After reading the tiff file all you need to do is append all images in one big array then you are good to go. Below is a small piece of code for your reference.
% fill up image_dimension and number of tiff images you have.
bigArray = zeros(image_dimension,numberImages)
for i=1:numberImages
t = Tiff('location of images','r');
bigArray(:,:,:,i) = read(t);
end
You need to change this code according to your problem statement.
I hope this helps.
Cheers.