center of gravity calculation from a dfiffraction limited spot

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Gift
Gift 2021-2-23
回答： darova 2021-3-2
I will like to calculate the center of gravity from arrays of spots from a png file. How can I go about it?
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Gift
Gift 2021-2-24
Thank you for your response. No, I did not use regionprops. Kindly find attached the image. If possible,I will also like to get the deviation of the spots.

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回答（2 个）

Bhomik Kankaria
Bhomik Kankaria 2021-2-27
Hi,
Center of gravity can be calculated using 'regionprops' function from Image Processing Toolbox. You can use centroid as property name to calculate center of gravity in a image.
To know more about the function regionprops, refer to the link below -
https://www.mathworks.com/help/images/ref/regionprops.html
  1 个评论
Gift
Gift 2021-3-1
Thank you for yoor rsponse. Please I am new to programming. can you kindly write a code on how it can be done. i.e the COG calculation and the lateral resolution

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darova
darova 2021-3-2
  • Try binarize or im2bw to convert image to 0-1 (binary). Experiment with grayscale parameter (see help)
  • use regionprops

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