Finding intensity along a moving line in an image and compiling to give an angle VS. Intensity plot
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I am currently beginning to work on a project to obtain the angle of collagen fiber orientation in tissue. This projects uses a technique called Small angle light scattering to give an image to be analyzed. From this image previous researchers such as Sacks Group in the USA. have used a technique where the following steps are completed:
- An image of the light scatter is obtained (Elliptical shape of white values on a black base)
- The major and minor axis are then placed on this area
- Then a circle is drawn around the center with the diameter of the major axis
- This circle is then traveled through with 1 degree increments with a line.
- At each of these increments an intensity value is found and recorded.
- These intensity values are stored and combined to give a final histogram of intensity values at each point
- This histogram allows for the angle be determined where the highest intensity is the dominant angle.
I was wondering is there anybody that may be able to help me determine how to obtain these intensities using mat lab. I would be very grateful for help on any of the above steps. Apologies for the poor description.
Thanks in Advance,
Regards
Thomas Sinnott
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Image Analyst
2013-5-29
Apologies won't solve your problem like a better description and an image will. Type alt-PrintScreen to copy your active window into the clipboard, then please post a few images to http://snag.gy so we can see what you're talking about. Then, is this tracking in 3D over multiple 2D images taken at different depths, where each image is of just a fiber cross section? Or is it of a single 2D image?
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Image Analyst
2013-7-3
Use improfile() to get the intensity profiles. Use the FAQ to get code to define the endpoints as they move along the circle. http://matlab.wikia.com/wiki/FAQ#How_do_I_create_a_circle.3F
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