About brightness

2011 5 23
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[EDIT: 20110523 17:15 CDT - reformat. clarify - WDR]
Hello everyone, I have a trouble with the brightness in a pic that I take:
http://i55.tinypic.com/2s1kt2g.jpg
I'm new in Matlab anyone can help me please? I need to remove the brightness in the edges, then to binarize just to see the white points, the picture is of bacterial colonies. Thanks!
PS: sorry for my bad English.

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Walter Roberson
Walter Roberson 2011-5-23

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I suggest im2bw perhaps in combination with graythresh

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