Hi Kalyani,
In order to outline and measure a monolayer boundary, you can use the bwboundaries function.
The documentation for the same is: https://www.mathworks.com/help/images/ref/bwboundaries.html.
Now before you go ahead and use the function, you need to ensure that you are doing all the necessary preprocessing before feeding the function the images you wanna measure the boundaries for.
Some examples of pre processing are smoothing and conversion to grayscale.
