a = imread('input.tif'); % convet uint8 to double a_double = double(a); % get number of rows and columns value [r,c] = size(a); % mean of gray scale image total_mean = sum(a_double(:))/(r*c); % variance of gray scale image total_difference = (a_double - total_mean).^2; total_sum = sum(total_difference(:)); limit = (r*c)-1; total_variance = total_sum/limit;
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