Compute thickness of a layer

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I would like to compute the thickness of a layer in an image (image_1.png). I would like to do this by computing the pixel value in the top layer subtracted by the pixel value in the corresponding bottom layer and averaged over the entire layer . Can someone help me how i could proceed with this?
Thank you
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Tri Rowstenkowski
Tri Rowstenkowski 2021-7-21
编辑:Tri Rowstenkowski 2021-7-28
The bright pixels together form a layer of the lumen (seen in image_4.jpg). I would like to compute its thickness(from green line to yellow line). Segmentation edges at pixel i, with the superscript top and bottom indicate the top and bottom edges, respectively and n is the total pixel number.
I would really appreciate your help in computing the thickness for image_2.png or image_3.png.

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