'Simple' object tracking on segmented microscopy images

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Nicolas Jaccard
Nicolas Jaccard 2013-9-30
评论： Nicolas Jaccard 2013-9-30
Dear all,
I have developed various microscopy image segmentation algorithms using MATLAB (mostly based on built-in functions from the Image Processing Toolbox).
The segmentation works very well for individual, standalone frames but I would like to use it to track movements of objects over time. More specifically, I would like to know when new objects appear, when 2 existing objects merge, etc. The objects move slowly and can easily be follow by eye when playing the movie.
I thought it would be a straightforward endeavour as I already have a very reliable segmentation algorithm. But I was unable to find an elegant way of doing it. I started playing with a crude approach that keeps track of the positions of the various objects overtime and detects events of note (merging, splitting, etc...). But I feel like I am reinventing the wheel and was wondering if there is more reliable and proven approach to such a problem.
Thanks in advance for any recommendation!
Nicolas

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Image Analyst
Image Analyst 2013-9-30
This kind of capability is in the Computer Vision System Toolbox. Have you checked out any of those demos and see if they can be applied to your videos?
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Anand
Anand 2013-9-30
In the tracking examples for CVST, you can replace the bounding box used for initialization with the bounding box for your segmented cell. For example, in this tracking example, replace bbox with the bounding box surrounding you're segmented cell.
Nicolas Jaccard
Nicolas Jaccard 2013-9-30
That makes sense. Thanks for the answer, will give it a go!

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