good day .. my aim is it extract optic disc ..i have applied otsu threshold method ..i have 2 subtract two images but when i subtract i get a blank image . my process followed green component -->tophat using large and small structural element that gives me 2 outputs --->then i apply otsu threshold for both tophat(large) and tophat(small) ..after this i want to subtract these otsu images ..but i am not able to do it please help me
se = strel('disk',500,8);
im2 = imtophat(I,se);
se1 = strel('disk',50,8);
im3 = imtophat(I,se1);
IDX = otsu(im2,6);
figure;
subplot(2,2,1);
axis image off;
imagesc(IDX)
colormap(gray);
IDX1 = otsu(im3,6);
figure;
subplot(2,2,1);
axis image off;
imagesc(IDX1)
colormap(gray);
i tried IDX -IDX1 but i amnot getting any image please somebody tell me whr am i goin wrong
