Clearing background of microscopy images

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Hello Everyone,
I have to measure the size of the blobs in the example image, but I cannot threshold them because some parts of the scratches have similar colors, so either I have to reduce the size of the blobs while thresholding, or I end up with a lot of smaller blobs from the scratches that end up skewing my distribution (like the second image). I have tried blurring the images and thresholding individual color channels (the scratches have higher average red values), but couldn't get a passable result without losing a lot of my actual features. Can someone point me towards a method/function that can help?
Thanks!

回答(1 个)

Seyeong Jeon
Seyeong Jeon 2023-6-28
I recommend you to add this line below your example result code.
image = imclose(image, ones(5));

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