Do you want two lines in one axes, or one line in each of two axes?
If two lines in one axes, it looks like you already have that, if the screen shot is what you have now.
If one line in each of two axes, create a second axes in App Designer or GUIDE, whichever you are using, and plot in each axes, e.g.:
CV1Plot=plot(handles.axes1,cv1(:,1),cv1(:,2),'b');
CV2Plot=plot(handles.axes2,cv2(:,1),cv2(:,2),'r');
"CV1Plot gives me the link to the same axes"
CV1Plot is a line in handles.axes1. In your example code, CV2Plot is a line in handles.axes1. In my example code above, CV2Plot is a line in handles.axes2.
"I try to create in gui graph with 2 plots in the same graphs."
If you are having trouble with that and are using App Designer (i.e., a uifigure), you need to specify the axes to hold in a uifigure, by default. That is:
CV1Plot=plot(handles.axes1,cv1(:,1),cv1(:,2),'b');
hold(handles.axes1,'on');
CV2Plot=plot(handles.axes1,cv2(:,1),cv2(:,2),'r');
hold(handles.axes1,'off');
However, the existence of the variable handles indicates you are likely using GUIDE (i.e., a figure), in which case specifying the axes to hold wouldn't usually be necessary. On the other hand, there's no reason you can't have a handles variable in App Designer, so who knows?
If these tips didn't sufficiently help, please clarify the situation: What are you using, GUIDE or App Designer? What exactly is your objective? What is the problem you are running into in trying to achieve that objective?